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Liquid Culture – Agrocybe aegerita

Liquid Culture – Agrocybe aegerita

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This is a mix of nutrients, desolved in water and pressure sterilized. Perfectly a monoculture is then innoculated, to prevent competition. Some species need extra nutrition, so the mycelium grows healthy prior to inoculation.
It is very effective and just a small amount is enought to inoculate a big amount of spawn or soil.

>>See all cultures we got for you right here<<

(Liquid, Petri, Slant, Grain, Dowel)

How to use mushroom liquid culture

  • Label the containers: Clearly label each container with the following information: date of preparation, mushroom species, and any other relevant details. Proper labeling will help you keep track of the cultures and their ages.
  • Store at the appropriate temperature: Most mushroom liquid cultures can be stored at cool temperatures, but the ideal range can vary depending on the species. A temperature range of 4°C to 10°C (39°F to 50°F) is generally suitable for many common mushroom species. This temperature slows down the growth of the culture, prolonging its viability.
  • Refresh the culture periodically: To maintain the culture’s health and vigor over an extended period, it’s a good idea to periodically transfer a portion of the liquid culture to fresh growth media. This process is known as subculturing and helps prevent genetic drift and the accumulation of harmful mutations.
  • Freezing can damage the delicate structures and cells of the mushrooms, leading to reduced viability and loss of growth potential.

Under ideal conditions, mushroom liquid cultures can remain viable and healthy for varying periods of time. Here are some general guidelines:

Short-term storage: If stored properly in a refrigerator at temperatures between 4°C to 10°C (39°F to 50°F), many mushroom liquid cultures can remain viable for a few weeks to a couple of months. Regularly inspect the cultures for signs of contamination or changes in appearance.

Medium-term storage: By transferring a small portion of the liquid culture to agar slants or Petri dishes, the viability can be extended. Agar cultures stored in the refrigerator can remain viable for several months to a year, depending on the species.

Long-term storage: For long-term storage, glycerol preservation or lyophilization (freeze-drying) are more suitable options. Glycerol-preserved cultures can be stored at ultra-low temperatures (e.g., -80°C) and may remain viable for several years. Freeze-dried cultures, when stored in a cool and dry place, can also last for years.

Transfer the Liquid Culture to the Substrate
Depending on the type of substrate, there are different methods for transferring the liquid culture. For example, if using grain as a substrate, you can mix the colonized liquid culture with sterilized grain.

Alow the Mycelium to Colonize the Substrate
After transferring the liquid culture maintain the right temperature, humidity, and light conditions. The colonization period can vary from a few weeks to several months, depending on the mushroom species and environmental factors.

What should i use liquid cultures for?

Supplement mushroom grain mycelium

Industries catering to the production of myco-products have harnessed the power of colonized mushroom grain mycelium as a valuable supplement. This enriched substrate offers a concentrated source of mycelial biomass and metabolites, which are sought-after for their nutritional and medicinal benefits. Most supplements you buy are done with only the mycelium of the mushroom.

Using it to grow actual mushrooms

Beyond supplement applications, colonized grain mycelium represents a crucial stage in the journey of mushroom cultivation. This potent mycelial network, teeming with life, is transferred onto suitable substrates such as straw, sawdust, or compost to initiate fruiting. Under controlled environmental conditions, the mycelium colonizes the new substrate, steadily working its way towards the production of fruiting bodies – the mushrooms.

What makes mushroom liquid culture so easy to use?

Mushroom liquid culture is considered easy to use for several reasons, making it a popular choice among mushroom cultivators:

  • Speed of Colonization: Liquid culture allows for faster colonization compared to solid substrates like agar or grain. The mycelium grows and spreads rapidly in the liquid medium, shortening the time required for the initial stages of the cultivation process.
  • Ease of Inoculation: Inoculating a liquid culture is straightforward and requires minimal equipment. You can introduce mushroom spores or mycelium into the liquid medium using a syringe or inoculation loop, and the mycelium starts growing without the need for additional steps.
  • Easy Transfer to Substrate: When the liquid culture is fully colonized, it can be easily transferred to a variety of substrates for fruiting. This transfer can be done without the need to handle delicate agar plates or grain jars, reducing the risk of contamination.
  • Reduced Contamination Risk: Since liquid culture is a closed system, it has a lower risk of contamination compared to open agar plates or grain jars. The liquid medium acts as a barrier, preventing external contaminants from entering.
  • Convenience for Research and Experimentation: For researchers or hobbyists working with multiple strains or species, liquid culture simplifies the process of maintaining and storing a diverse collection of mushroom cultures.

Our approach to your satisfaction

  • Clear Instructions: With every order, you will receive comprehensive and easy-to-follow instructions on handling and processing the cultures.
  • Complete Culture Kit: Choose us, and you’ll get all the items needed for a hassle-free growing experience, including 1x Self-Healing Injection Port, Air Filter, Closing Cone, Needle, and Alcohol Wipe.
  • Ensured Sterility and Freshness: We guarantee the highest level of sterility, providing fresh, contamination-free cultures that are ready to use upon arrival.
  • Responsive Customer Support: Our reliable customer support system promptly addresses any queries or issues you may have.
  • Educational Resources: Access our website’s abundance of educational resources, including cultivation guides, tips for successful growing, and troubleshooting solutions. We have gathered this information over years of experience, all available free of charge.
  • Secure Packaging and Shipping: To protect the cultures during shipping, we use sturdy and secure packaging.
  • Continued Support: Even after receiving your cultures, we offer post-purchase support, so you can seek guidance or ask questions whenever needed.

Questions? We´ve got you covered

A mushroom culture refers to a controlled growth of mushroom mycelium, the vegetative part of the fungus. It is typically grown on a suitable substrate and serves as the foundation for propagating and cultivating mushrooms.

To start a mushroom culture at home, you’ll need a clean and sterile environment. Inoculate a sterilized growth medium (such as agar or liquid broth) with mushroom spores or mycelium. Allow the mycelium to grow and expand in the medium, creating a colonized culture that can be used for further cultivation.

Liquid culture offers several advantages, including faster colonization, homogeneity, ease of transfer, and scalability. It is a versatile and efficient method for producing healthy mycelium for mushroom cultivation.

Maintaining sterility is crucial for successful mushroom culture. Work in a clean and sanitized environment, use proper sterile techniques, sterilize equipment, and handle cultures with care to prevent contamination.

Common issues in mushroom cultures include contamination, slow growth, and lack of fruiting. Troubleshoot these problems by identifying the cause (e.g., contamination source), adjusting environmental conditions, or using fresh and healthy cultures.

It is not recommended to use wild mushrooms for starting a culture due to potential contamination and uncertainty about the species. Instead, use commercial mushroom spores or mycelium from reputable sources.

It is not advisable to grow different mushroom species in the same culture jar, as they may compete or cross-contaminate. Each species should be cultured separately to maintain purity and avoid unwanted interactions.

Some beginner-friendly mushroom species include oyster mushrooms (Pleurotus spp.), Reishi mushrooms (Ganoderma spp.), and lion’s mane mushrooms (Hericium erinaceus). These species are relatively easy to cultivate and have broad environmental tolerances.

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