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Petri Dish – Inonotus obliquus

Petri Dish – Inonotus obliquus

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We will set a culture on a fresh Petri Dish for you. It will then grow out and if it withstands quality control, it will be sent. Petris are perfectly suited for short term storage, to multiply strains, to cross species and to clean up cultures. You may use a small cut of this cultures to innoculate substrates.

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(Liquid, Petri, Slant, Grain, Dowel)

How to use mushroom petri culture

  • Labeling: Properly label each petri dish with essential information such as the mushroom strain name, date of inoculation, and any relevant notes about the culture. Clear labeling helps to avoid confusion and ensures easy identification in the future.
  • Temperature: Store the labeled petri dishes in a controlled environment, preferably at a temperature between 4°C to 8°C (39°F to 46°F). This range maintains the mycelium’s stability without allowing it to grow too rapidly or deteriorate.
  • Refreshing: To extend the longevity of the mushroom petri culture, consider periodic subculturing. Transfer a small piece of healthy mycelium from the original petri dish to a fresh agar plate. This process helps rejuvenate the culture and prevent genetic degeneration over time.
  • Freezing: For long-term preservation, freezing can be an effective method. Prepare a cryoprotective solution using glycerol or dimethyl sulfoxide (DMSO) and add a few drops to the petri dish to protect the mycelium during freezing. Seal the petri dish and place it in a -80°C freezer or a liquid nitrogen container. When needed, thaw the frozen culture and transfer a piece of mycelium to a new agar plate for revival.
  • Short-term Cultures: If stored under appropriate conditions (around 4°C to 8°C or 39°F to 46°F), mushroom petri cultures can remain viable for several weeks to a few months. These short-term cultures are commonly used for immediate research, experimentation, or propagation.
  • Medium-term Cultures: With periodic subculturing (refreshing) onto fresh agar plates, mushroom petri cultures can be maintained for several months to a year. Regularly transferring a small piece of healthy mycelium to new agar helps prevent genetic degeneration and prolongs the culture’s viability.
  • Long-term Cultures: For long-term preservation, freezing is a preferred method. If stored at -80°C or in liquid nitrogen, mushroom petri cultures can remain viable for years or even decades. Cryoprotective agents, such as glycerol or DMSO, help protect the mycelium during freezing and thawing.
  1. Spawn Production
    Inoculation of Substrate: Prepare your chosen substrate, such as sterilized grains or sawdust, in a suitable container like a plastic bag or glass jar. Sterilize the substrate to eliminate competing organisms. Once cooled, use a sterile inoculation tool (e.g., scalpel, flame-sterilized needle) to transfer a small piece of healthy mycelium from the petri culture to the substrate.

    Incubation:
    Seal the container to maintain a controlled environment and promote mycelial growth. Keep the substrate in a warm, dark place for several days to allow the mycelium to colonize the substrate fully. This colonized substrate is now referred to as “spawn” and can be used to inoculate a larger growing medium.
  2. Cultivation
    Prepare the Growing Medium: Depending on the mushroom species, prepare a suitable growing medium, such as compost, pasteurized straw, or supplemented sawdust. Sterilize or pasteurize the medium to create an environment suitable for mushroom growth.
    Inoculate the Growing Medium: Use the spawn produced in the previous step to inoculate the growing medium. You can mix the spawn into the substrate or create layers with the spawn sandwiched between substrate layers. This process introduces the mycelium to the larger growing medium.Incubation and Fruiting: Place the inoculated growing medium in a controlled environment with proper humidity, temperature, and ventilation. The mycelium will continue to colonize the substrate, and eventually, mushrooms will start to form.
  3. Hybridization and Breeding
    Select Parental Strains: Choose two compatible mushroom strains that possess desirable traits you wish to combine in the offspring. These traits could include improved yield, size, flavor, or resistance to certain environmental conditions.Crossing and Petri Culture: In a sterile environment, take a small piece of mycelium from each parental strain and place them on a fresh petri dish. Allow them to grow and meet in the middle, where they will potentially fuse and form a hybrid mycelium.Isolation of Hybrid Mycelium: Once the hybrid mycelium has formed, isolate it from the parental strains by transferring a small piece of the hybrid mycelium to a new petri dish. This step ensures that you have a pure culture of the hybrid mycelium.

    Testing and Evaluation: Study the characteristics of the hybrid mycelium and observe its fruiting behavior. Evaluate whether the desired traits from both parental strains have been expressed in the offspring. e. Spawn Production and Cultivation: If the hybrid shows promising characteristics, proceed to spawn production and cultivation as mentioned earlier to grow the hybrid mushrooms on a larger scale.

What should i use petri cultures for?

Supplement mushroom grain mycelium

Industries catering to the production of myco-products have harnessed the power of colonized mushroom grain mycelium as a valuable supplement. This enriched substrate offers a concentrated source of mycelial biomass and metabolites, which are sought-after for their nutritional and medicinal benefits. Most supplements you buy are done with only the mycelium of the mushroom.

  • Research and Studies: Mushroom petri cultures serve as a basis for various research studies in mycology. They provide a controlled environment to study fungal growth, nutrition requirements, genetic characteristics, and response to different environmental conditions.
  • Spawn Production: Petri cultures are often used as the starting point for creating mushroom spawn. Spawn is the vegetative mycelium used to inoculate larger substrates, such as grain, sawdust, or compost, for mushroom cultivation.
  • Mushroom Cultivation: By transferring mycelium from petri cultures to appropriate substrates, cultivators can initiate mushroom fruiting. Petri cultures help ensure that the mushroom crop remains true to the desired strain and characteristics.
  • Hybridization and Breeding: In the field of mushroom breeding, petri cultures play a crucial role. Researchers can use different petri cultures to cross different strains and study the offspring for desired traits, leading to the development of new and improved varieties.
  • Pathogen Identification: In addition to beneficial mushrooms, petri cultures are used to identify and study fungal pathogens that may harm plants or other organisms. Identifying these pathogens is essential for managing and controlling diseases.

What makes mushroom liquid culture so easy to use?

Mushroom liquid culture is considered easy to use for several reasons, making it a popular choice among mushroom cultivators:

  • Speed of Colonization: Liquid culture allows for faster colonization compared to solid substrates like agar or grain. The mycelium grows and spreads rapidly in the liquid medium, shortening the time required for the initial stages of the cultivation process.
  • Ease of Inoculation: Inoculating a liquid culture is straightforward and requires minimal equipment. You can introduce mushroom spores or mycelium into the liquid medium using a syringe or inoculation loop, and the mycelium starts growing without the need for additional steps.
  • Easy Transfer to Substrate: When the liquid culture is fully colonized, it can be easily transferred to a variety of substrates for fruiting. This transfer can be done without the need to handle delicate agar plates or grain jars, reducing the risk of contamination.
  • Reduced Contamination Risk: Since liquid culture is a closed system, it has a lower risk of contamination compared to open agar plates or grain jars. The liquid medium acts as a barrier, preventing external contaminants from entering.
  • Convenience for Research and Experimentation: For researchers or hobbyists working with multiple strains or species, liquid culture simplifies the process of maintaining and storing a diverse collection of mushroom cultures.

Our approach to your satisfaction

  • Clear Instructions: With every order, you will receive comprehensive and easy-to-follow instructions on handling and processing the cultures.
  • Complete Culture Kit: Choose us, and you’ll get all the items needed for a hassle-free growing experience, including 1x Self-Healing Injection Port, Air Filter, Closing Cone, Needle, and Alcohol Wipe.
  • Ensured Sterility and Freshness: We guarantee the highest level of sterility, providing fresh, contamination-free cultures that are ready to use upon arrival.
  • Responsive Customer Support: Our reliable customer support system promptly addresses any queries or issues you may have.
  • Educational Resources: Access our website’s abundance of educational resources, including cultivation guides, tips for successful growing, and troubleshooting solutions. We have gathered this information over years of experience, all available free of charge.
  • Secure Packaging and Shipping: To protect the cultures during shipping, we use sturdy and secure packaging.
  • Continued Support: Even after receiving your cultures, we offer post-purchase support, so you can seek guidance or ask questions whenever needed.

Questions? We´ve got you covered

A mushroom culture refers to a controlled growth of mushroom mycelium, the vegetative part of the fungus. It is typically grown on a suitable substrate and serves as the foundation for propagating and cultivating mushrooms.

To start a mushroom culture at home, you’ll need a clean and sterile environment. Inoculate a sterilized growth medium (such as agar or liquid broth) with mushroom spores or mycelium. Allow the mycelium to grow and expand in the medium, creating a colonized culture that can be used for further cultivation.

Liquid culture offers several advantages, including faster colonization, homogeneity, ease of transfer, and scalability. It is a versatile and efficient method for producing healthy mycelium for mushroom cultivation.

Maintaining sterility is crucial for successful mushroom culture. Work in a clean and sanitized environment, use proper sterile techniques, sterilize equipment, and handle cultures with care to prevent contamination.

Common issues in mushroom cultures include contamination, slow growth, and lack of fruiting. Troubleshoot these problems by identifying the cause (e.g., contamination source), adjusting environmental conditions, or using fresh and healthy cultures.

It is not recommended to use wild mushrooms for starting a culture due to potential contamination and uncertainty about the species. Instead, use commercial mushroom spores or mycelium from reputable sources.

It is not advisable to grow different mushroom species in the same culture jar, as they may compete or cross-contaminate. Each species should be cultured separately to maintain purity and avoid unwanted interactions.

Some beginner-friendly mushroom species include oyster mushrooms (Pleurotus spp.), Reishi mushrooms (Ganoderma spp.), and lion’s mane mushrooms (Hericium erinaceus). These species are relatively easy to cultivate and have broad environmental tolerances.

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