You can use any typical decomposing growing methods (For example from our P.cubensis). But why should you do that, if you can set and forget for philosophers stones? Here we focus on how to grow the sclerotia. Mushrooms which are forming sclerotia: Psilocybe Mexicana, Psilocybe Tampanensis, Psilocybe Atl#7, Psilocybe Atlantis, Psilocybe Galindoi.
Growing Psilocybe tampanesis requires several steps to ensure successful cultivation. Here’s a comprehensive guide on how to grow these magic mushrooms.
Step 1: Spore Syringe or Liquid Mycelium for Grain Inoculation You have several options for inoculating your substrate. If you have a spore print of Psilocybe tampanesis or any other sclerotia-producing species, you can make a spore syringe. This involves preparing the syringe and rehydrating the spores for 1-2 days before inoculating the grain. Alternatively, you can create a liquid culture from a spore syringe or liquid mycelium, which allows for faster colonization and the ability to create more inoculation material.
Step 2: Choosing the Substrate The substrate is a crucial part of growing Psilocybe tampanesis. It’s recommended to use whole grain oats or ryegrass seeds as the substrate. Other options include rye berries, barley, brown rice flour, whole white/red millet, and wild bird seeds. It’s important to rinse the grains thoroughly and soak them in water for 24-48 hours at room temperature. Adding gypsum during soaking can help prevent clumping. After soaking, rinse the grains again and boil them for the appropriate amount of time.
Step 3: Jars or Bags for Cultivation For growing Psilocybe tampanesis, it’s best to use jars instead of bags. Jars provide better protection for the sensitive mycelium and help prevent contamination. Optimal jar sizes are between 16 ounces and 1 quart. You can fill the jars with the prepared substrate, leaving about 30-80% of the volume empty. Adding a layer of vermiculite at the bottom of each jar can help absorb excess moisture.
Step 4: Substrate Sterilization To ensure a sterile environment, cover each jar with aluminum foil and place them in a pressure cooker or autoclave. Sterilize the jars at the appropriate pressure and time, depending on their size. After sterilization, allow the jars to cool down to room temperature before proceeding.
Step 5: Inoculation Once the jars have cooled down, it’s time to inoculate them with either a spore syringe or liquid culture. Prepare a clean workspace and wear appropriate protective gear. Remove the foil from the jars, wipe the inoculation port with alcohol, and inject the spore or liquid culture into each jar. It’s crucial to maintain a sterile environment throughout this process to prevent contamination.
Step 6: Colonization Period After inoculation, place the jars in a dark location and allow the mycelium to colonize the substrate. The ideal temperature for colonization is around 70-75°F (21-24°C). If you want to speed up the process or if the room temperature is lower, you can use an incubator or an incubation chamber with temperature control. The colonization period typically takes 1-2 weeks, but it can vary.
Initiating Sclerotia Formation: Once the jars are fully colonized, induce sclerotia formation by exposing the mycelium to lower temperatures. Move the jars to a cooler environment, ideally around 60-68°F (16-20°C).
Wait for sclerotia growth: Over the course of several weeks, small, firm, brownish-colored sclerotia will form within the colonized grain jars. These sclerotia will resemble small stones or nuggets.
Harvest the sclerotia: Once the sclerotia have reached the desired size and hardness, they are ready for harvest. Carefully remove them from the grain jars and clean them to remove any excess substrate.
Store the harvested sclerotia: After cleaning, allow the harvested sclerotia to dry thoroughly. Once dry, store them in a cool, dry place in an airtight container to maintain their potency.
By following these steps, you can successfully grow Psilocybe tampanesis. Remember to maintain cleanliness and sterile conditions throughout the process to ensure the best results.