Summer to fall
Deciduous forests, spruce forests, forest meadows, rare in Western and Central Europe, from September to December.
Psilocybe serbica grows mainly in clumps, on well-decomposed conifers and deciduous trees, and together with Urtica spp. or rubus spp. on twigs, compost, plant debris, in woods, especially in moist places along streams, forest paths, and roadsides. It has not been reported to be synanthropic.
Preferred substrates are hardwood mulch made from oak, eucalyptus, Douglas fir, and alder. Fruiting occurs in cool weather, generally late September through January. The species can be easily grown on agar, grain seed, and cellulosic material, including wood chips and sawdust.